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  • Featured Product
  • KD/KO Validated

FTCD Monoclonal antibody

FTCD Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1H8A1

Cat No : 66979-1-Ig

Print datasheet

Synonyms

Glutamate formyltransferase, Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase

验证数据展示

  • IHC staining of human kidney using 66979-1-Ig

    IHC staining of human kidney using 66979-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FTCD antibody included
  • IHC staining of human kidney using 66979-1-Ig

    IHC staining of human kidney using 66979-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66979-1-Ig (FTCD antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FTCD antibody included
  • IF Staining of human liver cancer using 66979-1-Ig

    IF Staining of human liver cancer using 66979-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of mouse liver using 66979-1-Ig

    WB analysis of mouse liver using 66979-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66979-1-Ig (FTCD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of human liver cancer using 66979-1-Ig

    IF Staining of human liver cancer using 66979-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using FTCD antibody (66979-1-Ig, Clone: 1H8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue, pig kidney tissue, pig liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy FTCD Ready-To-Use IHC Kit. FTCD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66979-1-Ig targets FTCD in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse
Immunogen FTCD fusion protein Ag17429 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name formiminotransferase cyclodeaminase
Synonyms Glutamate formyltransferase, Glutamate formiminotransferase, Glutamate formimidoyltransferase, Formiminotransferase-cyclodeaminase, Formimidoyltransferase-cyclodeaminase
Calculated Molecular Weight 541 aa, 59 kDa
Observed Molecular Weight 53-62 kDa
GenBank Accession NumberBC136395
Gene Symbol FTCD
Gene ID (NCBI) 10841
RRIDAB_2882299
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95954
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine, a . This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).

实验方案

Product Specific Protocols
WB protocol for FTCD antibody 66979-1-IgDownload protocol
IHC protocol for FTCD antibody 66979-1-IgDownload protocol
IF protocol for FTCD antibody 66979-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Biomolecules

Exosome-Related FTCD Facilitates M1 Macrophage Polarization and Impacts the Prognosis of Hepatocellular Carcinoma

Authors - Youyi Liu
  • KD Validated
View Article