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PREB Monoclonal antibody

PREB Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F6A1

Cat no : 66981-1-Ig

Print datasheet

Synonyms

PREB, SEC12

验证数据展示

  • WB analysis of Daudi using 66981-1-Ig

    WB analysis of Daudi using 66981-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66981-1-Ig

    IHC staining of human prostate cancer using 66981-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PREB antibody included

经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PREB Ready-To-Use IHC Kit. PREB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66981-1-Ig targets PREB in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen PREB fusion protein Ag28549 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name prolactin regulatory element binding
Synonyms PREB, SEC12
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC002765
Gene Symbol PREB
Gene ID (NCBI) 10113
RRIDAB_2882301
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9HCU5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.

实验方案

Product Specific Protocols
WB protocol for PREB antibody 66981-1-IgDownload protocol
IHC protocol for PREB antibody 66981-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols