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TRAF5 Monoclonal antibody

TRAF5 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B1A7

Cat no : 66991-1-Ig

Print datasheet

Synonyms

RING finger protein 84, RNF84, TRAF5

验证数据展示

  • WB analysis of HeLa using 66991-1-Ig

    WB analysis of HeLa using 66991-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66991-1-Ig (TRAF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, LNCaP cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66991-1-Ig targets TRAF5 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen TRAF5 fusion protein Ag26070 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name TNF receptor-associated factor 5
Synonyms RING finger protein 84, RNF84, TRAF5
Calculated Molecular Weight 557 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC029600
Gene Symbol TRAF5
Gene ID (NCBI) 7188
RRIDAB_2882308
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00463
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRAF5 (also known as TNF receptor-associated factor 5, Ring finger protein 84, RNF8, MGC:39780) is a member of the tumor necrosis factor receptor-associated (TRAF) family of proteins that are characterized by the presence of a meprin and TRAF homology (MATH) domain, a RING-type zinc finger domain, and two TRAF-type zinc finger domains (https://www.uniprot.org/uniprot/O00463). TRAF5 is a scaffold protein that associates with and mediates signal transduction by cell surface receptors belonging to the tumor necrosis factor (TNF) superfamily, including CD27, CD30, CD40, and lymphotoxin-β receptor, positively regulating activation of the canonical NF-κB pathway and c-Jun NH2-terminal kinase (JNK) activation by these receptors (PMIDs: 8999898, 9582383, 8790348, 8663299). The RING-type zinc finger domain of TRAF5 exhibits ubiquitin protein ligase activity and is responsible for Lys-63-linked polyubiquitination of protein targets (PMIDs:23758787, 29176576). This domain has been demonstrated to be required for TRAF5-mediated activation of NF-κB (PMID:8663299).

实验方案

Product Specific Protocols
WB protocol for TRAF5 antibody 66991-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols