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验证数据展示

  • WB analysis using 66993-1-Ig

    WB analysis using 66993-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66993-1-Ig (UBAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 66993-1-Ig

    IHC staining of human heart using 66993-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human heart using 66993-1-Ig

    IHC staining of human heart using 66993-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66993-1-Ig (UBAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UBAP1 Monoclonal antibody

UBAP1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66993-1-Ig

产品说明书

CloneNo. 2A6A2

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

NAG20, UAP, UBAP, UBAP 1, UBAP1, ubiquitin associated protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 1900 μg/ml

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66993-1-Ig targets UBAP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 UBAP1 fusion protein Ag17675 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin associated protein 1
别名 NAG20, UAP, UBAP, UBAP 1, UBAP1, ubiquitin associated protein 1
计算分子量 502 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC020950
基因名称 UBAP1
Gene ID (NCBI) 51271
RRIDAB_2882310
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NZ09
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.

实验方案

Product Specific Protocols
WB protocol for UBAP1 antibody 66993-1-IgDownload protocol
IHC protocol for UBAP1 antibody 66993-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols