• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human placenta using 67030-1-Ig

    WB analysis of human placenta using 67030-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 67030-1-Ig

    IHC staining of human spleen using 67030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 67030-1-Ig

    IHC staining of human spleen using 67030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 67030-1-Ig

    IF Staining of human tonsillitis using 67030-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PRTN3 antibody (67030-1-Ig, Clone: 2D1B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 67030-1-Ig

    IF Staining of human tonsillitis using 67030-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using PRTN3 antibody (67030-1-Ig, Clone: 2D1B8 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PR3/PRTN3 Monoclonal antibody

PR3/PRTN3 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67030-1-Ig

产品说明书

CloneNo. 2D1B8

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

PRTN3, NP-4, Myeloblastin, MBN, EC:3.4.21.76

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67030-1-Ig targets PR3/PRTN3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 PR3/PRTN3 fusion protein Ag25915 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 proteinase 3
别名 PRTN3, NP-4, Myeloblastin, MBN, EC:3.4.21.76
计算分子量 256 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC096183
基因名称 PRTN3
Gene ID (NCBI) 5657
RRIDAB_2882345
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP24158
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PR3/PRTN3 antibody 67030-1-IgDownload protocol
IHC protocol for PR3/PRTN3 antibody 67030-1-IgDownload protocol
IF protocol for PR3/PRTN3 antibody 67030-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols