验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, mouse brain tissue, pig brain tissue, mouse cerebellum tissue |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67062-1-Ig targets PDE4D in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Immunogen | PDE4D fusion protein Ag8302 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila) |
| Synonyms | PDE43, EC:3.1.4.53, DPDE3, cAMP-specific phosphodiesterase 4D, 3',5'-cyclic-AMP phosphodiesterase 4D |
| Calculated Molecular Weight | 809 aa, 91 kDa |
| Observed Molecular Weight | 91-110 kDa, 66-84 kDa |
| GenBank Accession Number | BC036319 |
| Gene Symbol | PDE4D |
| Gene ID (NCBI) | 5144 |
| RRID | AB_2882372 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q08499 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also known as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram (PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in the hippocampus from a patient with advanced Alzheimer disease (PMID:17353396). Bands of 90-110 kDa correspond to the long PDE4D isoforms, and bands of 66-84 kDa are the short PDE4D isoforms (PMID: 17215491).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PDE4D antibody 67062-1-Ig | Download protocol |
| IF protocol for PDE4D antibody 67062-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
