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验证数据展示

  • WB analysis using 67069-2-Ig

    WB analysis using 67069-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67069-2-Ig (ESD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67069-2-Ig

    IF Staining of HEK-293 using 67069-2-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ESD antibody (67069-2-Ig, Clone: 2F5E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

ESD Monoclonal antibody

ESD Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67069-2-Ig

产品说明书

CloneNo. 2F5E1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, Caco-2 cells, HepG2 cells, Jurkat cells, K-562 cells, Ramos cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67069-2-Ig targets ESD in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ESD fusion protein Ag7487 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 esterase D/formylglutathione hydrolase
别名 Methylumbelliferyl-acetate deacetylase, FGH, Esterase D, EC:3.1.2.12, EC:3.1.1.56
计算分子量 31 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC001169
基因名称 ESD
Gene ID (NCBI) 2098
RRIDAB_3670378
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP10768
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Esterase D (ESD) is a non-specific esterase widely distributed in various organisms and is also named S-Formylglutathione Hydrolase (SFGH). ESD is a member of the carboxylesterase family and has both carboxylesterase and thioesterase activities. ESD plays an important role in the process of glutathione-dependent detoxification, regulating cholesterol efflux and virus infection in humans, and is closely related to the development of tumors. ESD as a Genetic Marker for Retinoblastoma (PMID: 32247735, PMID: 34875997, PMID: 35627173). The calculated molecular weight of ESD is 31 kDa.

实验方案

Product Specific Protocols
WB protocol for ESD antibody 67069-2-IgDownload protocol
IF protocol for ESD antibody 67069-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols