验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, A549 cells, NCI-H1299 cells, Caco-2 cells, HT-29 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, HepG2 cells, K-562 cells, PC-12 cells, Neuro-2a cells |
| Positive IP detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67109-1-Ig targets CNBP in WB, IF, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CNBP fusion protein Ag7114 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CCHC-type zinc finger, nucleic acid binding protein |
| 别名 | CNBP, CNBP1, DM2, FLJ11631, PROMM, RNF163, ZCCHC22, Zinc finger protein 9, ZNF9 |
| 计算分子量 | 19 kDa |
| 观测分子量 | 19-25 kDa |
| GenBank蛋白编号 | BC000288 |
| 基因名称 | CNBP |
| Gene ID (NCBI) | 7555 |
| RRID | AB_2882413 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P62633 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNBP antibody 67109-1-Ig | Download protocol |
| IP protocol for CNBP antibody 67109-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Microbiol The SARS-CoV-2 RNA-protein interactome in infected human cells.
| ||
PLoS One CircPRMT5 promotes progression of osteosarcoma by recruiting CNBP to regulate the translation and stability of CDK6 mRNA | ||
J Mol Cell Cardiol Cellular nucleic acid binding protein facilitates cardiac repair after myocardial infarction by activating β-catenin signaling | ||
PLoS One circTADA2A inhibited SLC38A1 expression and suppresses melanoma progression through the prevention of CNBP trans-activation | ||
bioRxiv A KIF1C-CNBP motor-adaptor complex for trafficking mRNAs to cell protrusions
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