验证数据展示
经过测试的应用
| Positive WB detected in | Mo7e cells, THP-1 cells, U-937 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67135-2-Ig targets CD33 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | CD33 fusion protein Ag11402 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD33 molecule |
| 别名 | CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3 |
| 计算分子量 | 364 aa, 40 kDa |
| 观测分子量 | 67-70 kDa |
| GenBank蛋白编号 | BC028152 |
| 基因名称 | CD33 |
| Gene ID (NCBI) | 945 |
| RRID | AB_3085036 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P20138 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD33 antibody 67135-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
