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验证数据展示

  • IHC staining of human breast cancer using 67144-1-Ig

    IHC staining of human breast cancer using 67144-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with URG4 antibody included
  • IHC staining of human breast cancer using 67144-1-Ig

    IHC staining of human breast cancer using 67144-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67144-1-Ig (URG4 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with URG4 antibody included
  • WB analysis using 67144-1-Ig

    WB analysis using 67144-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67144-1-Ig (URG4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67144-1-Ig

    IF Staining of HeLa using 67144-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using URG4 antibody (67144-1-Ig, Clone: 2D5F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

URG4 Monoclonal antibody

URG4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67144-1-Ig

产品说明书

CloneNo. 2D5F12

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KIAA1507, up regulated gene 4, URG4, URGCP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSGC-7901 cells, MKN-45 cells, HepG2 cells, HuH-7 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human nasopharyngeal carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy URG4 Ready-To-Use IHC Kit. URG4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67144-1-Ig targets URG4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 URG4 fusion protein Ag28635 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 up-regulated gene 4
别名 KIAA1507, up regulated gene 4, URG4, URGCP
计算分子量 922 aa, 104 kDa
观测分子量 104 kDa
GenBank蛋白编号BC018426
基因名称 URG4
Gene ID (NCBI) 55665
RRIDAB_2882443
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TCY9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

URG4 (upregulated gene 4), also known as upregulator of cell proliferation (URGCP), is an oncogene which is involved in the development and progression of various tumors. URG4 is upregulated in hepatocellular carcinoma (HCC), ovarian cancer, and other types of cancer. Overexpression of URG4 not only results in tumour progression but also in metastasis and recurrence.

实验方案

Product Specific Protocols
WB protocol for URG4 antibody 67144-1-IgDownload protocol
IHC protocol for URG4 antibody 67144-1-IgDownload protocol
IF protocol for URG4 antibody 67144-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols