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HSPA4 Monoclonal antibody

HSPA4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F2C12

Cat no : 67173-1-Ig

Print datasheet

Synonyms

APG 2, APG2, Heat shock 70 kDa protein 4, heat shock 70kDa protein 4, HS24/P52, HSP70, hsp70RY, HSPA4, RY

验证数据展示

  • WB analysis using 67173-1-Ig

    WB analysis using 67173-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67173-1-Ig (HSPA4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67173-1-Ig

    WB analysis of HEK-293 using 67173-1-Ig

    WB result of HSPA4 antibody (67173-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA4 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, pig brain tissue, HepG2 cells, L02 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67173-1-Ig targets HSPA4 in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, pig
Immunogen HSPA4 fusion protein Ag16624 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 4
Synonyms APG 2, APG2, Heat shock 70 kDa protein 4, heat shock 70kDa protein 4, HS24/P52, HSP70, hsp70RY, HSPA4, RY
Calculated Molecular Weight 840 aa, 94 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC110861
Gene Symbol HSPA4
Gene ID (NCBI) 3308
RRIDAB_2882469
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP34932
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPA4 (also known as Apg-2, HSP70RY) is a 110 kDa cytosolic protein, a member of the heat-shock protein 110 (Hsp 110) subfamily of Hsp70 proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA4 is ubiquitously expressed and its expression is not heat inducible. Overexpression of HSPA4 has been reported in some leukemia and solid tumors.

实验方案

Product Specific Protocols
WB protocol for HSPA4 antibody 67173-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols