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验证数据展示

  • WB analysis using 67181-1-Ig

    WB analysis using 67181-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67181-1-Ig (GAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 67181-1-Ig

    IHC staining of human gliomas using 67181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 67181-1-Ig

    IHC staining of human gliomas using 67181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GAS1 Monoclonal antibody

GAS1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67181-1-Ig

产品说明书

CloneNo. 2H8E1

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Pig

应用

WB, IHC, ELISA

别名

GAS 1, GAS1, growth arrest 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells, pig brain tissue, Y79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67181-1-Ig targets GAS1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse, Pig
免疫原 GAS1 fusion protein Ag12259 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 growth arrest-specific 1
别名 GAS 1, GAS1, growth arrest 1
计算分子量 345 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC074908
基因名称 GAS1
Gene ID (NCBI) 2619
RRIDAB_2882477
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54826
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAS1 is involved in growth suppression. It is an integral plasma membrane protein whose expression is linked to growth arrest. It can be used as a novel therapeutic candidate for gastric cancer.

实验方案

Product Specific Protocols
WB protocol for GAS1 antibody 67181-1-IgDownload protocol
IHC protocol for GAS1 antibody 67181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols