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NUFIP2 Monoclonal antibody

NUFIP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4E3

Cat no : 67195-1-Ig

Print datasheet

Synonyms

Cell proliferation-inducing gene 1 protein, 82-FIP, 82 kDa FMRP-interacting protein, 82 FIP, 2A4E3

验证数据展示

  • WB analysis using 67195-1-Ig

    WB analysis using 67195-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 67195-1-Ig

    IHC staining of mouse cerebellum using 67195-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUFIP2 antibody included
  • IHC staining of mouse cerebellum using 67195-1-Ig

    IHC staining of mouse cerebellum using 67195-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NUFIP2 antibody included
  • IF Staining of HepG2 using 67195-1-Ig

    IF Staining of HepG2 using 67195-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUFIP2 antibody (67195-1-Ig, Clone: 2A4E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inHeLa cells, 4T1 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inmouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NUFIP2 Ready-To-Use IHC Kit. NUFIP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67195-1-Ig targets NUFIP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen NUFIP2 fusion protein Ag29040 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nuclear fragile X mental retardation protein interacting protein 2
Synonyms Cell proliferation-inducing gene 1 protein, 82-FIP, 82 kDa FMRP-interacting protein, 82 FIP, 2A4E3
Calculated Molecular Weight 695 aa, 76 kDa
Observed Molecular Weight 76 kDa, 82 kDa
GenBank Accession NumberBC108307
Gene Symbol NUFIP2
Gene ID (NCBI) 57532
RRIDAB_2882489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z417
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NUFIP2 antibody 67195-1-IgDownload protocol
IHC protocol for NUFIP2 antibody 67195-1-IgDownload protocol
IF protocol for NUFIP2 antibody 67195-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols