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验证数据展示

  • WB analysis using 67214-1-Ig

    WB analysis using 67214-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67214-1-Ig (EIF5A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67214-1-Ig

    IF Staining of HEK-293 using 67214-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF5A antibody (67214-1-Ig, Clone: 2D1B6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

EIF5A Monoclonal antibody

EIF5A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67214-1-Ig

产品说明书

CloneNo. 2D1B6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A, eIF 4D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, LNCaP cells, Jurkat cells, NIH/3T3 cells, HEK-293 cells, PC-3 cells, HSC-T6 cells, RAW 264.7 cells, HaCaT cells, HepG2 cells, SK-BR-3 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy EIF5A Ready-To-Use IHC Kit. EIF5A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67214-1-Ig targets EIF5A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EIF5A fusion protein Ag28588 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 5A
别名 eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A, eIF 4D
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号BC001832
基因名称 EIF5A
Gene ID (NCBI) 1984
RRIDAB_2882506
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP63241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.

实验方案

Product Specific Protocols
WB protocol for EIF5A antibody 67214-1-IgDownload protocol
IF protocol for EIF5A antibody 67214-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols