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CYP11A1 Monoclonal antibody
CYP11A1 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1C5D5
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HepG2 cells, SiHa cells, HeLa cells, Caco-2 cells. human placenta tissue, human testis tissue, rat testis tissue, mouse testis tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 3 publications below |
产品信息
67264-1-Ig targets CYP11A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | CYP11A1 fusion protein Ag4353 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cytochrome P450, family 11, subfamily A, polypeptide 1 |
| Synonyms | Cytochrome P450 11A1, CYPXIA1, CYP11A, Cholesterol side-chain cleavage enzyme, mitochondrial, Cholesterol desmolase |
| Calculated Molecular Weight | 521 aa, 60 kDa |
| Observed Molecular Weight | 49-52 kDa |
| GenBank Accession Number | BC032329 |
| Gene Symbol | CYP11A1 |
| Gene ID (NCBI) | 1583 |
| RRID | AB_2882535 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05108 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP11A1 antibody 67264-1-Ig | Download protocol |
| IHC protocol for CYP11A1 antibody 67264-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Neurosci Autism-Like Behavior in the Offspring of CYP11A1-Overexpressing Pregnant Rats. | ||
Reprod Sci Identification of Key Differentially Methylated/Expressed Genes and Pathways for Ovarian Endometriosis by Bioinformatics Analysis | ||
Toxicol Appl Pharmacol Reproductive effects of pubertal exposure to neonicotinoid thiacloprid in immature male mice | ||
Mol Hum Reprod Reduced oxygen concentrations regulate the phenotype and function of human granulosa cells in vitro and cause a diminished steroidogenic but increased inflammatory cellular reaction |