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验证数据展示

  • WB analysis of T-47D using 67269-1-Ig

    WB analysis of T-47D using 67269-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67269-1-Ig (PSG1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 67269-1-Ig

    IHC staining of human placenta using 67269-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 67269-1-Ig

    IHC staining of human placenta using 67269-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67269-1-Ig (PSG1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 67269-1-Ig

    IF Staining of human placenta using 67269-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using 67269-1-Ig

    IF Staining of human placenta using 67269-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PSG1 antibody (67269-1-Ig, Clone: 1C6C4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSG1 Monoclonal antibody

PSG1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67269-1-Ig

产品说明书

CloneNo. 1C6C4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

Fetal liver non-specific cross-reactive antigen 1/2, DHFRP2, CD66f, CD66 antigen-like family member F, B1G1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inT-47D cells, L02 cells, NCCIT cells, JAR cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67269-1-Ig targets PSG1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 PSG1 fusion protein Ag28604 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 pregnancy specific beta-1-glycoprotein 1
别名 Fetal liver non-specific cross-reactive antigen 1/2, DHFRP2, CD66f, CD66 antigen-like family member F, B1G1
计算分子量 426 aa, 48 kDa
观测分子量 54-70 kDa
GenBank蛋白编号BC022338
基因名称 PSG1
Gene ID (NCBI) 5669
RRIDAB_2882539
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP11464
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSG1 antibody 67269-1-IgDownload protocol
IHC protocol for PSG1 antibody 67269-1-IgDownload protocol
IF protocol for PSG1 antibody 67269-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols