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验证数据展示

  • WB analysis using 67287-1-Ig

    WB analysis using 67287-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 67287-1-Ig

    IHC staining of human skin cancer using 67287-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67287-1-Ig (DAAM1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

DAAM1 Monoclonal antibody

DAAM1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67287-1-Ig

产品说明书

CloneNo. 3F11F11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KIAA0666, FLJ41657, Disheveled-associated activator of morphogenesis 1, 3F11F11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, rat heart tissue, mouse heart tissue, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67287-1-Ig targets DAAM1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DAAM1 fusion protein Ag6809 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 dishevelled associated activator of morphogenesis 1
别名 KIAA0666, FLJ41657, Disheveled-associated activator of morphogenesis 1, 3F11F11
计算分子量 123 kDa
观测分子量 120 kDa
GenBank蛋白编号BC064999
基因名称 DAAM1
Gene ID (NCBI) 23002
RRIDAB_2882553
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y4D1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.

实验方案

Product Specific Protocols
WB protocol for DAAM1 antibody 67287-1-IgDownload protocol
IHC protocol for DAAM1 antibody 67287-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols