• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67323-1-Ig

    WB analysis using 67323-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 67323-1-Ig

    IHC staining of mouse cerebellum using 67323-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 67323-1-Ig

    IHC staining of mouse cerebellum using 67323-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 67323-1-Ig

    IF Staining of mouse brain using 67323-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 67323-1-Ig

    IF Staining of mouse brain using 67323-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Alpha SNAP Monoclonal antibody

Alpha SNAP Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67323-1-Ig

产品说明书

CloneNo. 1E2A2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Pig, Mouse, Rat

应用

WB, IHC, IF-P, ELISA

别名

Alpha SNAP, NAPA, SNAP alpha, SNAPA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inpig brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67323-1-Ig targets Alpha SNAP in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, Pig, Mouse, Rat
免疫原 Alpha SNAP fusion protein Ag13180 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 N-ethylmaleimide-sensitive factor attachment protein, alpha
别名 Alpha SNAP, NAPA, SNAP alpha, SNAPA
计算分子量 33 kDa
观测分子量 34 kDa
GenBank蛋白编号BC007432
基因名称 Alpha SNAP
Gene ID (NCBI) 8775
RRIDAB_2882583
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54920
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha SNAP antibody 67323-1-IgDownload protocol
IHC protocol for Alpha SNAP antibody 67323-1-IgDownload protocol
IF protocol for Alpha SNAP antibody 67323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols