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Alpha SNAP Monoclonal antibody

Alpha SNAP Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E2A2

Cat no : 67323-1-Ig

Print datasheet

Synonyms

Alpha SNAP, NAPA, SNAP alpha, SNAPA

验证数据展示

  • WB analysis using 67323-1-Ig

    WB analysis using 67323-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67323-1-Ig (alpha-SNAP antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 67323-1-Ig

    IHC staining of mouse cerebellum using 67323-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 67323-1-Ig

    IHC staining of mouse cerebellum using 67323-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67323-1-Ig (Alpha SNAP antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 67323-1-Ig

    IF Staining of mouse brain using 67323-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 67323-1-Ig

    IF Staining of mouse brain using 67323-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Alpha SNAP antibody (67323-1-Ig, Clone: 1E2A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig brain tissue, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67323-1-Ig targets Alpha SNAP in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Pig, Mouse, Rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Pig, Mouse, Rat
Immunogen Alpha SNAP fusion protein Ag13180 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name N-ethylmaleimide-sensitive factor attachment protein, alpha
Synonyms Alpha SNAP, NAPA, SNAP alpha, SNAPA
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC007432
Gene Symbol Alpha SNAP
Gene ID (NCBI) 8775
RRIDAB_2882583
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54920
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha SNAP antibody 67323-1-IgDownload protocol
IHC protocol for Alpha SNAP antibody 67323-1-IgDownload protocol
IF protocol for Alpha SNAP antibody 67323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols