HSP20 Monoclonal antibody
HSP20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, IF-P, ELISA
Conjugate
Unconjugated
CloneNo.
1G7D2
验证数据展示
经过测试的应用
| Positive WB detected in | pig skeletal muscle tissue, mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | U-118 MG cells |
Planning an IHC experiment? We recommend our IHCeasy HSPB6 Ready-To-Use IHC Kit. HSPB6 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:850-1:3400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
67327-1-Ig targets HSP20 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Immunogen | HSP20 fusion protein Ag10484 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | heat shock protein, alpha-crystallin-related, B6 |
| Synonyms | HspB6, Heat shock protein family B member 6, Heat shock protein beta-6, Heat shock protein beta 6, Heat shock 20 kDa-like protein p20 |
| Calculated Molecular Weight | 160 aa, 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC068046 |
| Gene Symbol | HSP20 |
| Gene ID (NCBI) | 126393 |
| RRID | AB_2882586 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O14558 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP20 antibody 67327-1-Ig | Download protocol |
| IHC protocol for HSP20 antibody 67327-1-Ig | Download protocol |
| IF protocol for HSP20 antibody 67327-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|