• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ODC1 Monoclonal antibody

ODC1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

2A1E3

Cat No : 67336-1-Ig

Print datasheet

Synonyms

ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1

验证数据展示

  • WB analysis of MCF-7 using 67336-1-Ig

    WB analysis of MCF-7 using 67336-1-Ig

    WB result of ODC1 antibody (67336-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ODC1 transfected MCF-7 cells.

  • WB analysis of MCF-7 using 67336-1-Ig

    WB analysis of MCF-7 using 67336-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67336-1-Ig (ODC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inMCF-7 cells, HUVEC cells, human placenta tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67336-1-Ig targets ODC1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB,ELISA Application Description
Tested Reactivity Human
Immunogen ODC1 fusion protein Ag10927 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ornithine decarboxylase 1
Synonyms ODC, ODC1, Ornithine decarboxylase, ornithine decarboxylase 1
Calculated Molecular Weight 461 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC025296
Gene Symbol ODC1
Gene ID (NCBI) 4953
RRIDAB_2882594
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11926
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ornithine decarboxylase (ODC) is also named as ODC1 and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control (PMID:8486633). This protein can be phosphorylated in vivo (PMID:8798774). ODC1 can form a homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers (PMID: 10623504). The molecular mass of ODC1 is 51 kDa, and the homodimer is 106 kDa.

实验方案

Product Specific Protocols
WB protocol for ODC1 antibody 67336-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols