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FUT3 Monoclonal antibody

FUT3 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A6G7

Cat no : 67344-1-Ig

Print datasheet

Synonyms

EC:2.4.1.-, CD174, Blood group Lewis alpha-4-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase

验证数据展示

  • WB analysis of human placenta using 67344-1-Ig

    WB analysis of human placenta using 67344-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of human prostate hyperplasia using 67344-1-Ig

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human prostate hyperplasia using 67344-1-Ig

    IF Staining of human prostate hyperplasia using 67344-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human colon cancer using 67344-1-Ig

    IHC staining of human colon cancer using 67344-1-Ig

    Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman placenta tissue, NCCIT cells, JAR cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate hyperplasia tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67344-1-Ig targets FUT3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen FUT3 fusion protein Ag12581 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
Synonyms EC:2.4.1.-, CD174, Blood group Lewis alpha-4-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase
Calculated Molecular Weight 361 aa, 42 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC108675
Gene Symbol FUT3
Gene ID (NCBI) 2525
RRIDAB_2882602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP21217
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FUT3 antibody 67344-1-IgDownload protocol
IHC protocol for FUT3 antibody 67344-1-IgDownload protocol
IF protocol for FUT3 antibody 67344-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols