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PTPN2 Monoclonal antibody

PTPN2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A1D1

Cat no : 67388-1-Ig

Print datasheet

Synonyms

PTPN2, PTPT, TC PTP, TCELLPTP, TCPTP

验证数据展示

  • WB analysis using 67388-1-Ig

    WB analysis using 67388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67388-1-Ig

    WB analysis using 67388-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67388-1-Ig

    IHC staining of human breast cancer using 67388-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN2 antibody included
  • IHC staining of human breast cancer using 67388-1-Ig

    IHC staining of human breast cancer using 67388-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PTPN2 antibody included

经过测试的应用

Positive WB detected inPC-3 cells, HeLa cells, 4T1 cells, LNCaP cells, HEK-293T cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PTPN2 Ready-To-Use IHC Kit. PTPN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67388-1-Ig targets PTPN2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen PTPN2 fusion protein Ag29749 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 2
Synonyms PTPN2, PTPT, TC PTP, TCELLPTP, TCPTP
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC016727
Gene Symbol PTPN2
Gene ID (NCBI) 5771
RRIDAB_2882632
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP17706
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.

实验方案

Product Specific Protocols
WB protocol for PTPN2 antibody 67388-1-IgDownload protocol
IHC protocol for PTPN2 antibody 67388-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols