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  • Featured Product
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LACTB2 Monoclonal antibody

LACTB2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1G6B1

Cat no : 67399-1-Ig

Print datasheet

Synonyms

Beta lactamase like protein 2, CGI 83, lactamase, beta 2, LACTB2

验证数据展示

  • WB analysis of A549 using 67399-1-Ig

    WB analysis of A549 using 67399-1-Ig

    WB result of LACTB2 antibody (67399-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LACTB2 transfected A549 cells.

  • WB analysis using 67399-1-Ig

    WB analysis using 67399-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67399-1-Ig (LACTB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67399-1-Ig

    IHC staining of human liver cancer using 67399-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67399-1-Ig

    IHC staining of human liver cancer using 67399-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67399-1-Ig (LACTB2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human liver cancer using 67399-1-Ig

    IF Staining of human liver cancer using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 67399-1-Ig

    IF Staining of human liver cancer using 67399-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using LACTB2 antibody (67399-1-Ig, Clone: 1G6B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67399-1-Ig targets LACTB2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen LACTB2 fusion protein Ag10231 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lactamase, beta 2
Synonyms Beta lactamase like protein 2, CGI 83, lactamase, beta 2, LACTB2
Calculated Molecular Weight 288 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC000878
Gene Symbol LACTB2
Gene ID (NCBI) 51110
RRIDAB_2882642
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ53H82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LACTB2 antibody 67399-1-IgDownload protocol
IHC protocol for LACTB2 antibody 67399-1-IgDownload protocol
IF protocol for LACTB2 antibody 67399-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols