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验证数据展示

  • WB analysis using 67402-1-Ig

    WB analysis using 67402-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67402-1-Ig

    IHC staining of human liver cancer using 67402-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AGXT antibody included
  • IHC staining of human kidney using 67402-1-Ig

    IHC staining of human kidney using 67402-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AGXT antibody included

AGXT Monoclonal antibody

AGXT Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67402-1-Ig

产品说明书

CloneNo. 3E12H1

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, NCI-H1299 cells, A549 cells, L02 cells, HuH-7 cells, SMMC-7721 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AGXT Ready-To-Use IHC Kit. AGXT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67402-1-Ig targets AGXT in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 AGXT fusion protein Ag19188 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 alanine-glyoxylate aminotransferase
别名 AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6
计算分子量 392 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC132819
基因名称 AGXT
Gene ID (NCBI) 189
RRIDAB_2882644
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21549
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.

实验方案

Product Specific Protocols
WB protocol for AGXT antibody 67402-1-IgDownload protocol
IHC protocol for AGXT antibody 67402-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols