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GRIM19 Monoclonal antibody, PBS Only (Capture/Detector)

GRIM19 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A10C5

Cat no : 67414-1-PBS

Print datasheet

Synonyms

NDUFA13, CI B16.6, CGI-39, CGI 39, Cell death regulatory protein GRIM-19

验证数据展示

  • WB analysis of LNCaP using 67414-1-Ig (same clone as 67414-1-PBS)

    WB analysis of LNCaP using 67414-1-Ig (same clone as 67414-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67414-1-Ig (same clone as 67414-1-PBS)

    WB analysis of HepG2 using 67414-1-Ig (same clone as 67414-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67414-1-Ig (GRIM19 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA13 antibody included
  • IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    IHC staining of human liver cancer using 67414-1-Ig (same clone as 67414-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67414-1-Ig (GRIM19 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67414-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NDUFA13 antibody included
  • Cytometric bead array standard curve of MP50735-1

    Cytometric bead array standard curve of MP50735-1

    Cytometric bead array standard curve of MP50735-1, GRIM19 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67414-2-PBS. Detection antibody: 67414-1-PBS. Standard:Ag20855. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50735-3

    Cytometric bead array standard curve of MP50735-3

    Cytometric bead array standard curve of MP50735-3, GRIM19 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67414-1-PBS. Detection antibody: 67414-4-PBS. Standard:Ag20855. Range: 0.098-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy NDUFA13 Ready-To-Use IHC Kit. NDUFA13 primary antibody included.

产品信息

67414-1-PBS targets GRIM19 as part of a matched antibody pair:

MP50735-1: 67414-2-PBS capture and 67414-1-PBS detection (validated in Cytometric bead array)

MP50735-3: 67414-1-PBS capture and 67414-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen GRIM19 fusion protein Ag20855 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
Synonyms NDUFA13, CI B16.6, CGI-39, CGI 39, Cell death regulatory protein GRIM-19
Calculated Molecular Weight16.5 kDa
Observed Molecular Weight 16-18 kDa
GenBank Accession NumberBC009189
Gene Symbol GRIM19
Gene ID (NCBI) 51079
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9P0J0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).