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验证数据展示

  • WB analysis using 67416-1-Ig (same clone as 67416-1-PBS)

    WB analysis using 67416-1-Ig (same clone as 67416-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67416-1-Ig (ERO1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67416-1-Ig (same clone as 67416-1-PBS)

    WB analysis of HepG2 using 67416-1-Ig (same clone as 67416-1-PBS)

    WB result of ERO1L antibody (67416-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERO1L transfected HepG2 cells. This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    IHC staining of human pancreas cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    IHC staining of human pancreas cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67416-1-Ig (ERO1L antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • IF Staining of human stomach cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    IF Staining of human stomach cancer using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using ERO1L antibody (67416-1-Ig, Clone: 1G12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67416-1-Ig (same clone as 67416-1-PBS)

    IF Staining of HEK-293 using 67416-1-Ig (same clone as 67416-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using ERO1L antibody (67416-1-Ig, Clone: 1G12E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67416-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51017-4

    Cytometric bead array standard curve of MP51017-4

    Cytometric bead array standard curve of MP51017-4, ERO1L Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67416-1-PBS. Detection antibody: 67416-5-PBS. Standard:Ag29910. Range: 3.125-100 ng/mL.

  • Featured Product
  • KD/KO验证

ERO1L Monoclonal antibody, PBS Only (Capture)

ERO1L Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 67416-1-PBS

产品说明书

CloneNo. 1G12E11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

别名

ERO1 L, ERO1 alpha, Endoplasmic reticulum oxidoreductase alpha, Endoplasmic oxidoreductin-1-like protein, EC:1.8.4.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67416-1-PBS targets ERO1L as part of a matched antibody pair:

MP51017-4: 67416-1-PBS capture and 67416-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ERO1L fusion protein Ag29910 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ERO1-like (S. cerevisiae)
别名 ERO1 L, ERO1 alpha, Endoplasmic reticulum oxidoreductase alpha, Endoplasmic oxidoreductin-1-like protein, EC:1.8.4.-
计算分子量 468 aa, 54 kDa
观测分子量 54 kDa
GenBank蛋白编号BC008674
基因名称 ERO1L
Gene ID (NCBI) 30001
RRIDAB_2882656
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96HE7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ERO1L, also named as ERO1-alpha, is an essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. It acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. It does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Glutathione may be required to regulate its activity in the endoplasmic reticulum. It may be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. It is required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. ERO1L has a calculated molecular weight of 54 kDa and can be detected as 60kDa.