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验证数据展示

  • WB analysis using 67450-1-Ig

    WB analysis using 67450-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67450-1-Ig (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67450-1-Ig

    WB analysis of HEK-293 using 67450-1-Ig

    WB result of HSP90AB1 antibody (67450-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

HSP90AB1 Monoclonal antibody

HSP90AB1 Monoclonal Antibody for WB, ELISA
Cat No. 67450-1-Ig

产品说明书

CloneNo. 2D3D1
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat and More (1)

应用

WB, ELISA

别名

D6S182, FLJ26984, Heat shock 84 kDa, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP84, HSP90 BETA, HSP90AB1, HSP90B, HSPC2, HSPCB

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, Neuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy HSP90AB1 Ready-To-Use IHC Kit. HSP90AB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67450-1-Ig targets HSP90AB1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse, rat
文献引用反应性human
免疫原 HSP90AB1 fusion protein Ag28379 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock protein 90kDa alpha (cytosolic), class B member 1
别名 D6S182, FLJ26984, Heat shock 84 kDa, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP84, HSP90 BETA, HSP90AB1, HSP90B, HSPC2, HSPCB
计算分子量 723 aa, 83 kDa
观测分子量 83-90 kDa
GenBank蛋白编号BC014485
基因名称 HSP90AB1
Gene ID (NCBI) 3326
RRIDAB_2882684
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08238
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers.

实验方案

Product Specific Protocols
WB protocol for HSP90AB1 antibody 67450-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Virol Sin

Hsp90 β is Critical for the Infection of Severe Fever with Thrombocytopenia Syndrome Virus

Authors - Bo Wang
  • KD Validated
View Article