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HMBS Monoclonal antibody

HMBS Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G12H3

Cat no : 67456-1-Ig

Print datasheet

Synonyms

HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS

验证数据展示

  • WB analysis using 67456-1-Ig

    WB analysis using 67456-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67456-1-Ig (HMBS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67456-1-Ig

    IHC staining of human breast cancer using 67456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67456-1-Ig

    IHC staining of human breast cancer using 67456-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67456-1-Ig (HMBS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells, HepG2 cells, Caco-2 cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67456-1-Ig targets HMBS in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen HMBS fusion protein Ag6594 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hydroxymethylbilane synthase
Synonyms HMBS, hydroxymethylbilane synthase, PBG D, PBGD, Porphobilinogen deaminase, Pre uroporphyrinogen synthase, UPS
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-42 kDa
GenBank Accession NumberBC000520
Gene Symbol HMBS
Gene ID (NCBI) 3145
RRIDAB_2882688
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08397
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HMBS antibody 67456-1-IgDownload protocol
IHC protocol for HMBS antibody 67456-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols