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PSMG3 Monoclonal antibody

PSMG3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H9C3

Cat no : 67466-1-Ig

Print datasheet

Synonyms

C7orf48, hPAC3, PAC 3, PAC3, PSMG3

验证数据展示

  • WB analysis using 67466-1-Ig

    WB analysis using 67466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67466-1-Ig

    WB analysis using 67466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67466-1-Ig (PSMG3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 67466-1-Ig

    IF Staining of HeLa using 67466-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMG3 antibody (67466-1-Ig, Clone: 2H9C3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, K-562 cells, HL-60 cells, A549 cells, Hela cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67466-1-Ig targets PSMG3 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen PSMG3 fusion protein Ag8384 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name proteasome (prosome, macropain) assembly chaperone 3
Synonyms C7orf48, hPAC3, PAC 3, PAC3, PSMG3
Calculated Molecular Weight 13 kDa
Observed Molecular Weight13 kDa
GenBank Accession NumberBC004308
Gene Symbol PSMG3
Gene ID (NCBI) 84262
RRIDAB_2882695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BT73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PSMG3 antibody 67466-1-IgDownload protocol
IF protocol for PSMG3 antibody 67466-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols