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验证数据展示

  • WB analysis using 67490-1-Ig

    WB analysis using 67490-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67490-1-Ig (UNG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 67490-1-Ig

    WB analysis of HepG2 using 67490-1-Ig

    WB result of UNG antibody (67490-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UNG transfected HepG2 cells.

  • IHC staining of human liver cancer using 67490-1-Ig

    IHC staining of human liver cancer using 67490-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67490-1-Ig (UNG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with UNG antibody included
  • Featured Product
  • KD/KO验证

UNG Monoclonal antibody

UNG Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67490-1-Ig

产品说明书

CloneNo. 2B3B2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

DGU, DKFZp781L1143, HIGM4, UDG, UNG, UNG1, UNG15, UNG2, uracil DNA glycosylase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LNCaP cells, HT-29 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UNG Ready-To-Use IHC Kit. UNG primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67490-1-Ig targets UNG in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 UNG fusion protein Ag29979 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 uracil-DNA glycosylase
别名 DGU, DKFZp781L1143, HIGM4, UDG, UNG, UNG1, UNG15, UNG2, uracil DNA glycosylase
计算分子量 304 aa, 34 kDa
观测分子量34 kDa
GenBank蛋白编号BC015205
基因名称 UNG
Gene ID (NCBI) 7374
RRIDAB_2918492
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13051
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UNG antibody 67490-1-IgDownload protocol
IHC protocol for UNG antibody 67490-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols