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CMPK1 Monoclonal antibody

CMPK1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11G7

Cat no : 67519-1-Ig

Print datasheet

Synonyms

CMK, CMPK, CMPK1, Cytidine monophosphate kinase, Cytidylate kinase, Deoxycytidylate kinase, UCK, UMK, UMP CMP kinase, UMP CMPK, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate kinase

验证数据展示

  • WB analysis using 67519-1-Ig

    WB analysis using 67519-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67519-1-Ig (CMPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67519-1-Ig

    IF Staining of HeLa using 67519-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CMPK1 antibody (67519-1-Ig, Clone: 2D11G7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CMPK1 Ready-To-Use IHC Kit. CMPK1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67519-1-Ig targets CMPK1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen CMPK1 fusion protein Ag30084 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
Synonyms CMK, CMPK, CMPK1, Cytidine monophosphate kinase, Cytidylate kinase, Deoxycytidylate kinase, UCK, UMK, UMP CMP kinase, UMP CMPK, UMP/CMP kinase, UMP/CMPK, UMPK, Uridine monophosphate kinase
Calculated Molecular Weight 228 aa, 26 kDa
Observed Molecular Weight 22-26 kDa
GenBank Accession NumberBC014961
Gene Symbol CMPK1
Gene ID (NCBI) 51727
RRIDAB_2882739
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30085
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.

实验方案

Product Specific Protocols
WB protocol for CMPK1 antibody 67519-1-IgDownload protocol
IF protocol for CMPK1 antibody 67519-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols