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FES Monoclonal antibody

FES Monoclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1E1B11

Cat No : 67524-1-Ig

Print datasheet

Synonyms

feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps

验证数据展示

  • WB analysis using 67524-1-Ig

    WB analysis using 67524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67524-1-Ig (FES antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 67524-1-Ig

    IF Staining of A549 using 67524-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 67524-1-Ig (FES antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inTHP-1 cells, TF-1 cells, HL-60 cells, U-937 cells
Positive IF detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67524-1-Ig targets FES in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, WB,ELISA Application Description
Tested Reactivity Human
Immunogen FES fusion protein Ag28108 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name feline sarcoma oncogene
Synonyms feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps
Calculated Molecular Weight 822 aa, 94 kDa
Observed Molecular Weight 93 kDa, 79 kDa, 87 kDa, 85 kDa
GenBank Accession NumberBC035357
Gene Symbol FES
Gene ID (NCBI) 2242
RRIDAB_2882744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07332
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FES antibody 67524-1-IgDownload protocol
IF protocol for FES antibody 67524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols