• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FES Monoclonal antibody

FES Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1B11

Cat no : 67524-1-Ig

Print datasheet

Synonyms

Proto oncogene c Fes, p93c-fes, FPS, Feline sarcoma/Fujinami avian sarcoma oncogene homolog, EC:2.7.10.2

验证数据展示

  • WB analysis using 67524-1-Ig

    WB analysis using 67524-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67524-1-Ig (FES antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 67524-1-Ig

    IF Staining of A549 using 67524-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 67524-1-Ig (FES antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inTHP-1 cells, Karpas-299 cells, NR8383 cells, RAW 264.7 cells, TF-1 cells, HL-60 cells, U-937 cells
Positive IF/ICC detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67524-1-Ig targets FES in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen FES fusion protein Ag28108 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name feline sarcoma oncogene
Synonyms Proto oncogene c Fes, p93c-fes, FPS, Feline sarcoma/Fujinami avian sarcoma oncogene homolog, EC:2.7.10.2
Calculated Molecular Weight 822 aa, 94 kDa
Observed Molecular Weight 93 kDa, 79 kDa, 87 kDa, 85 kDa
GenBank Accession NumberBC035357
Gene Symbol FES
Gene ID (NCBI) 2242
RRIDAB_2882744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07332
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for FES antibody 67524-1-IgDownload protocol
IF protocol for FES antibody 67524-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols