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Species Reactivity
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验证数据展示

  • WB analysis using 67536-1-Ig

    WB analysis using 67536-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67536-1-Ig (RAB8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67536-1-Ig

    IF Staining of HepG2 using 67536-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RAB8A antibody (67536-1-Ig, Clone: 1D12G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

RAB8A Monoclonal antibody

RAB8A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67536-1-Ig

产品说明书

CloneNo. 1D12G4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

1D12G4, EC:3.6.5.2, MEL, Oncogene c mel, Oncogene c-mel

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67536-1-Ig targets RAB8A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RAB8A, member RAS oncogene family
别名 1D12G4, EC:3.6.5.2, MEL, Oncogene c mel, Oncogene c-mel
计算分子量 24 kDa
观测分子量 24 kDa
GenBank蛋白编号NM_005370
基因名称 RAB8A
Gene ID (NCBI) 4218
RRIDAB_2882755
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP61006
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAB8A antibody 67536-1-IgDownload protocol
IF protocol for RAB8A antibody 67536-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols