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  • Featured Product
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RBM8A,Y14 Monoclonal antibody

RBM8A,Y14 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F5G1

Cat no : 67541-1-Ig

Print datasheet

Synonyms

RBM8A, BOV 1C, BOV 1B, BOV 1A, BOV 1

验证数据展示

  • WB analysis using 67541-1-Ig

    WB analysis using 67541-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human ovary tumor using 67541-1-Ig

    IHC staining of human ovary tumor using 67541-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBM8A antibody included
  • WB analysis using 67541-1-Ig

    WB analysis using 67541-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67541-1-Ig (RBM8A,Y14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67541-1-Ig

    WB analysis of HeLa using 67541-1-Ig

    WB result of RBM8A,Y14 antibody (67541-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

  • IF Staining of A549 using 67541-1-Ig

    IF Staining of A549 using 67541-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using RBM8A,Y14 antibody (67541-1-Ig, Clone: 1F5G1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy RBM8A Ready-To-Use IHC Kit. RBM8A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67541-1-Ig targets RBM8A,Y14 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen RBM8A,Y14 fusion protein Ag6800 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RNA binding motif protein 8A
Synonyms RBM8A, BOV 1C, BOV 1B, BOV 1A, BOV 1
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC017088
Gene Symbol RBM8A
Gene ID (NCBI) 9939
RRIDAB_2882760
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y5S9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBM8A,Y14 antibody 67541-1-IgDownload protocol
IHC protocol for RBM8A,Y14 antibody 67541-1-IgDownload protocol
IF protocol for RBM8A,Y14 antibody 67541-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity

Authors - Dan Li
View Article