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Septin 9 Monoclonal antibody

Septin 9 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C6F10

Cat no : 67548-1-Ig

Print datasheet

Synonyms

AF17q25, FLJ75490, KIAA0991, MLL septin like fusion protein, MSF, MSF1, NAPB, Ov/Br septin, Ovarian/Breast septin, PNUTL4, SEPT9, SeptD1, Septin 9, Septin D1, SINT1

验证数据展示

  • WB analysis using 67548-1-Ig

    WB analysis using 67548-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67548-1-Ig (Septin 9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67548-1-Ig

    WB analysis using 67548-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67548-1-Ig (Septin 9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat colon using 67548-1-Ig

    WB analysis of rat colon using 67548-1-Ig

    rat colon tissue were subjected to SDS PAGE followed by western blot with 67548-1-Ig (Septin 9 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inpig colon tissue, A549 cells, rat colon tissue, MCF-7 cells, HeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SEPTIN9 Ready-To-Use IHC Kit. SEPTIN9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67548-1-Ig targets Septin 9 in WB, ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, pig, rat
Immunogen Septin 9 fusion protein Ag18047 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name septin 9
Synonyms AF17q25, FLJ75490, KIAA0991, MLL septin like fusion protein, MSF, MSF1, NAPB, Ov/Br septin, Ovarian/Breast septin, PNUTL4, SEPT9, SeptD1, Septin 9, Septin D1, SINT1
Calculated Molecular Weight 64 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC021192
Gene Symbol SEPT9
Gene ID (NCBI) 10801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UHD8
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).

实验方案

Product Specific Protocols
WB protocol for Septin 9 antibody 67548-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols