验证数据展示
经过测试的应用
| Positive WB detected in | pig colon tissue, A549 cells, rat colon tissue, MCF-7 cells, HeLa cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SEPTIN9 Ready-To-Use IHC Kit. SEPTIN9 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67548-1-Ig targets Septin 9 in WB, ELISA applications and shows reactivity with Human, pig, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, pig, rat |
| 免疫原 | Septin 9 fusion protein Ag18047 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | septin 9 |
| 别名 | AF17q25, FLJ75490, KIAA0991, MLL septin like fusion protein, MSF, MSF1, NAPB, Ov/Br septin, Ovarian/Breast septin, PNUTL4, SEPT9, SeptD1, Septin 9, Septin D1, SINT1 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC021192 |
| 基因名称 | SEPT9 |
| Gene ID (NCBI) | 10801 |
| RRID | AB_2935251 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UHD8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SEPT9 belongs to the septin family which is a group of highly conserved GTP-binding proteins in eukaryotes. SEPT9 plays a role in cytokinesis, the final stage of cell division. SEPT9 gene methylation has been implicated as a biomarker for colorectal cancer(CRC).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 9 antibody 67548-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |