SUMO1 Monoclonal antibody
SUMO1 Monoclonal Antibody for WB, IF/ICC, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat and More (1)
Applications
WB, IF/ICC, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3B5B5
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IHC detected in | mouse kidney tissue, rat kidney tissue, rat small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:800-1:3200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IP | See 1 publications below |
产品信息
67559-1-Ig targets SUMO1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | WB, IF/ICC, IHC, ELISA Application Description |
Cited Applications | IP |
Tested Reactivity | Human, Mouse, Rat |
Cited Reactivity | mouse |
Immunogen | SUMO1 fusion protein Ag29402 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) |
Synonyms | DAP 1, GAP modifying protein 1, GMP1, OFC10, PIC1, Sentrin, SMT3, SMT3 homolog 3, SMT3C, SMT3H3, SUMO 1, SUMO1, Ubiquitin like protein SMT3C, Ubiquitin like protein UBL1, UBL1 |
Calculated Molecular Weight | 12 kDa |
Observed Molecular Weight | 15-100 kDa |
GenBank Accession Number | BC006462 |
Gene Symbol | SUMO1 |
Gene ID (NCBI) | 7341 |
RRID | AB_2882773 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P63165 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SUMO1 antibody 67559-1-Ig | Download protocol |
IHC protocol for SUMO1 antibody 67559-1-Ig | Download protocol |
IF protocol for SUMO1 antibody 67559-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Biomed Pharmacother Hydrogen sulfide regulates SERCA2a SUMOylation by S-Sulfhydration of SENP1 to ameliorate cardiac systole-diastole function in diabetic cardiomyopathy |