CD63 Monoclonal antibody
CD63 Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (3)
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3D4D1
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, U2OS cells, MCF-7 cells, A549 cells, K-562 cells, HL-60, THP-1 cells, LNCaP cells, HUVEC cells |
Positive IHC detected in | human tonsillitis tissue, human malignant melanoma tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue, human malignant melanoma tissue, human lymphoma tissue |
Positive FC detected in | THP-1 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:350-1:1400 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67605-1-Ig targets CD63 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | FC, IF-P, IHC, WB, ELISA |
Cited Applications | WB, FC |
Tested Reactivity | Human |
Cited Reactivity | human, rat, goat |
Immunogen | CD63 fusion protein Ag19690 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | CD63 molecule |
Synonyms | CD63, CD63 antigen, CD63 molecule, Granulophysin, ME491, MLA1, OMA81H, Tetraspanin 30, Tspan 30, TSPAN30 |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 35 kDa |
GenBank Accession Number | BC002349 |
Gene Symbol | CD63 |
Gene ID (NCBI) | 967 |
RRID | AB_2882811 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08962 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD63 antibody 67605-1-Ig | Download protocol |
IHC protocol for CD63 antibody 67605-1-Ig | Download protocol |
IF protocol for CD63 antibody 67605-1-Ig | Download protocol |
FC protocol for CD63 antibody 67605-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Lactate dehydrogenase A regulates tumor-macrophage symbiosis to promote glioblastoma progression | ||
Drug Des Devel Ther Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling | ||
Oxid Med Cell Longev Adipose-Derived Circulating Exosomes Promote Protection of the Pulmonary Endothelial Barrier by Inhibiting EndMT and Oxidative Stress through Down-Regulation of the TGF-β Pathway: A Potential Explanation for the Obesity Paradox in ARDS. | ||
Aging (Albany NY) MSC-derived exosomes promote recovery from traumatic brain injury via microglia/macrophages in rat. | ||
Aging (Albany NY) Exosomal circRNAs contribute to intestinal development via the VEGF signalling pathway in human term and preterm colostrum. | ||
Aging (Albany NY) Endothelial cell-derived exosomal circHIPK3 promotes the proliferation of vascular smooth muscle cells induced by high glucose via the miR-106a-5p/Foxo1/Vcam1 pathway. |