验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67621-1-Ig targets TAF12 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 | TAF12 fusion protein Ag16597 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa |
| 别名 | TAF12, TAF15, TAF2J, TAFII 20/TAFII 15, TAFII20, TAFII20/TAFII15 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC011986 |
| 基因名称 | TAF12 |
| Gene ID (NCBI) | 6883 |
| RRID | AB_2882823 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q16514 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF12 antibody 67621-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |