验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Hela cells, HepG2 cells, Jurkat cells, K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
产品信息
67625-1-Ig targets SORD in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, rat, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | SORD fusion protein Ag8651 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | sorbitol dehydrogenase |
| 别名 | L iditol 2 dehydrogenase, SDH, sorbitol dehydrogenase, SORD, SORD1 |
| 计算分子量 | 357 aa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC021085 |
| 基因名称 | SORD |
| Gene ID (NCBI) | 6652 |
| RRID | AB_2882826 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q00796 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SORD(Sorbitol dehydrogenase) is also named as L-iditol 2-dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. It catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase, makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications. This protein can form a homotetramer(PMID:12962626). Measurement of SORD is a specific indicator of liver cell damage and parenchymal.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SORD antibody 67625-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Nutr Erythritol synthesis is elevated in response to oxidative stress and regulated by the non-oxidative pentose phosphate pathway in A549 cells
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