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Ch-TOG Monoclonal antibody, PBS Only (Capture/Detector)

Ch-TOG Monoclonal Antibody for WB, IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

1H5G1

Cat No : 67631-1-PBS

Print datasheet

Synonyms

ch TOG, CHTOG, Ch-TOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp

验证数据展示

  • WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

    WB analysis using 67631-1-Ig (same clone as 67631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67631-1-Ig (CKAP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67631-1-Ig (same clone as 67631-1-PBS)

    IF Staining of HepG2 using 67631-1-Ig (same clone as 67631-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Ch-TOG antibody (67631-1-Ig, Clone: 1H5G1 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67631-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50224-1

    Cytometric bead array standard curve of MP50224-1

    Cytometric bead array standard curve of MP50224-1, CKAP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67631-2-PBS. Detection antibody: 67631-1-PBS. Standard:Ag28770. Range: 0.391-100 ng/mL.

  • Cytometric bead array standard curve of MP50224-2

    Cytometric bead array standard curve of MP50224-2

    Cytometric bead array standard curve of MP50224-2, CKAP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67631-1-PBS. Detection antibody: 67631-3-PBS. Standard:Ag28770. Range: 6.25-100 ng/mL.

产品信息

67631-1-PBS targets Ch-TOG as part of a matched antibody pair:

MP50224-1: 67631-2-PBS capture and 67631-1-PBS detection (validated in Cytometric bead array)

MP50224-2: 67631-1-PBS capture and 67631-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen Ch-TOG fusion protein Ag28770 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytoskeleton associated protein 5
Synonyms ch TOG, CHTOG, Ch-TOG, CKAP5, FLJ35359, KIAA0097, MSPS, TOG, TOGp
Observed Molecular Weight 220-240 kDa
GenBank Accession NumberBC120869
Gene Symbol CKAP5
Gene ID (NCBI) 9793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14008
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Ch-TOG (colonic hepatic tumor-overexpressed gene), also known as XMAP215 or CKAP5, is a microtubule polymerase which can promotes cytoplasmic microtubule nucleation and elongation. Through interacting with Aurora-A and TACC3, it plays a major role in organizing spindle poles.