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验证数据展示

  • WB analysis using 67658-1-Ig

    WB analysis using 67658-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 67658-1-Ig

    FC experiment of A549 using 67658-1-Ig

    1x10^6 A549 cells were intracellularly stained with 0.4 ug PROCR Monoclonal antibody (67658-1-Ig, Clone:1F5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human tonsillitis using 67658-1-Ig

    IHC staining of human tonsillitis using 67658-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67658-1-Ig

    IHC staining of human tonsillitis using 67658-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPCR/CD201 Monoclonal antibody

EPCR/CD201 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67658-1-Ig

产品说明书

CloneNo. 1F5C4
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, pig and More (1)

应用

WB, IHC, FC (Intra), ELISA and More (1)

别名

CD201, EPCR, PROCR, 1F5C4, Activated protein C receptor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, pig spleen tissue, pig heart tissue, pig vein tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67658-1-Ig targets EPCR/CD201 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, pig
文献引用反应性human, mouse
免疫原 EPCR/CD201 fusion protein Ag25628 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein C receptor, endothelial (EPCR)
别名 CD201, EPCR, PROCR, 1F5C4, Activated protein C receptor
计算分子量 238 aa, 27 kDa
观测分子量49 kDa
GenBank蛋白编号BC014451
基因名称 PROCR
Gene ID (NCBI) 10544
RRIDAB_2882855
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UNN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EPCR/CD201 antibody 67658-1-IgDownload protocol
IHC protocol for EPCR/CD201 antibody 67658-1-IgDownload protocol
FC protocol for EPCR/CD201 antibody 67658-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Signal Transduct Target Ther

Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.

Authors - Panpan Zhang
View Article