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RNMT Monoclonal antibody

RNMT Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3F1C9

Cat no : 67673-1-Ig

Print datasheet

Synonyms

DKFZp686H1252, hcm1p, hCMT1, hCMT1c, hMet, KIAA0398, MET, mRNA cap methyltransferase, RG7MT1, RNMT

验证数据展示

  • WB analysis using 67673-1-Ig

    WB analysis using 67673-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67673-1-Ig (RNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67673-1-Ig targets RNMT in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen RNMT fusion protein Ag6953 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RNA (guanine-7-) methyltransferase
Synonyms DKFZp686H1252, hcm1p, hCMT1, hCMT1c, hMet, KIAA0398, MET, mRNA cap methyltransferase, RG7MT1, RNMT
Calculated Molecular Weight 476 aa, 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC036798
Gene Symbol RNMT
Gene ID (NCBI) 8731
RRIDAB_2918497
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43148
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNMT, also named as mRNA cap guanine-N7 methyltransferase or KIAA0398, is a 476 amino acid protein, which contains one mRNA cap 0 methyltransferase domain and belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. RNMT localizes in the nucleus and is widely expressed in various tissues. RNMT as a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs and binds RNA containing 5'-terminal GpppC.

实验方案

Product Specific Protocols
WB protocol for RNMT antibody 67673-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols