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RPS12 Monoclonal antibody

RPS12 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3D1

Cat no : 67683-1-Ig

Print datasheet

Synonyms

40S ribosomal protein S12, ribosomal protein S12, RPS12

验证数据展示

  • WB analysis using 67683-1-Ig

    WB analysis using 67683-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67683-1-Ig (RPS12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67683-1-Ig

    IHC staining of human breast cancer using 67683-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67683-1-Ig (RPS12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67683-1-Ig

    IF Staining of HepG2 using 67683-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS12 antibody (67683-1-Ig, Clone: 1A3D1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, NCI-H1299 cells, HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, PC-12cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67683-1-Ig targets RPS12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen RPS12 fusion protein Ag9649 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ribosomal protein S12
Synonyms 40S ribosomal protein S12, ribosomal protein S12, RPS12
Calculated Molecular Weight 132 aa, 15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC017321
Gene Symbol RPS12
Gene ID (NCBI) 6206
RRIDAB_2882876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP25398
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.

实验方案

Product Specific Protocols
WB protocol for RPS12 antibody 67683-1-IgDownload protocol
IHC protocol for RPS12 antibody 67683-1-IgDownload protocol
IF protocol for RPS12 antibody 67683-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols