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ALPK1 Monoclonal antibody

ALPK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B8B5

Cat no : 67689-1-Ig

Print datasheet

Synonyms

Chromosome 4 kinase, ALPK, Alpha-protein kinase 1, Alpha protein kinase 1, alpha kinase 1

验证数据展示

  • WB analysis using 67689-1-Ig

    WB analysis using 67689-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67689-1-Ig (ALPK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 67689-1-Ig

    IHC staining of human heart using 67689-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67689-1-Ig (ALPK1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 67689-1-Ig

    IF Staining of THP-1 using 67689-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using ALPK1 antibody (67689-1-Ig, Clone: 2B8B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inCOLO 320 cells, T-47D cells, A549 cells, U-87 MG cells, Daudi cells, THP-1 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67689-1-Ig targets ALPK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ALPK1 fusion protein Ag5320 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name alpha-kinase 1
Synonyms Chromosome 4 kinase, ALPK, Alpha-protein kinase 1, Alpha protein kinase 1, alpha kinase 1
Calculated Molecular Weight 139 kDa
Observed Molecular Weight139 kDa
GenBank Accession NumberBC060780
Gene Symbol ALPK1
Gene ID (NCBI) 80216
RRIDAB_2882882
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96QP1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-protein kinase 1, also known as alpha-kinase 1 (ALPK1), is associated with chronic kidney disease (CKD), myocardial infarction, gout and type 2 diabetes mellitus (DM). ALPK1 mutant has been reported to cause severe defects in motor co‐ordination in mice. ALPK1 enhances the expression of proinflammatory cytokines including IL-1β, IL-8 and TGF-β1 in cultured human THP1 and human embryonic kidney 293 (HEK293) cells (PMID: 31557402, PMID: 26275947). ALPK1 short isoform (108 kDa) was highly expressed in brain, spinal cord, heart, lung, spleen, thymus, small intestine, skin and testis, while detectable inskeletal muscle and kidney. ALPK1 large isoform (130 kDa) appeared in heart, lung, thymus and skin (PMID: 21208416).

实验方案

Product Specific Protocols
WB protocol for ALPK1 antibody 67689-1-IgDownload protocol
IHC protocol for ALPK1 antibody 67689-1-IgDownload protocol
IF protocol for ALPK1 antibody 67689-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols