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FABP2 Monoclonal antibody

FABP2 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2D11G6

Cat no : 67691-1-Ig

Print datasheet

Synonyms

FABP2, FABPI, Fatty acid binding protein 2, I FABP

验证数据展示

  • WB analysis using 67691-1-Ig

    WB analysis using 67691-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse colon using 67691-1-Ig

    IF Staining of mouse colon using 67691-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using FABP2 antibody (67691-1-Ig, Clone: 2D11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis of COLO 320 using 67691-1-Ig

    WB analysis of COLO 320 using 67691-1-Ig

    WB result of FABP2 antibody (67691-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP2 transfected COLO 320 cells.

  • IHC staining of mouse small intestine using 67691-1-Ig

    IHC staining of mouse small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FABP2 antibody included
  • IHC staining of mouse small intestine using 67691-1-Ig

    IHC staining of mouse small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FABP2 antibody included
  • IHC staining of rat small intestine using 67691-1-Ig

    IHC staining of rat small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FABP2 antibody included
  • IHC staining of human small intestine using 67691-1-Ig

    IHC staining of human small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FABP2 antibody included
  • IHC staining of human small intestine using 67691-1-Ig

    IHC staining of human small intestine using 67691-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with FABP2 antibody included

经过测试的应用

Positive WB detected inrat small intestine tissue, human jejunum tissue, COLO 320 cells, pig duodenum, mouse small intestine, rabbit small intestine
Positive IHC detected inmouse small intestine tissue, mouse colon tissue, rat small intestine tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue
Planning an IHC experiment? We recommend our IHCeasy FABP2 Ready-To-Use IHC Kit. FABP2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67691-1-Ig targets FABP2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat, rabbit, pig
Cited Reactivitymouse
Immunogen FABP2 fusion protein Ag17620 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name fatty acid binding protein 2, intestinal
Synonyms FABP2, FABPI, Fatty acid binding protein 2, I FABP
Calculated Molecular Weight 132 aa, 15 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC069617
Gene Symbol FABP2
Gene ID (NCBI) 2169
RRIDAB_2882884
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP12104
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FABP2, also known as the intestinal fatty acid binding protein (I-FABP), is expressed in the absorptive intestinal villus cells. It is mainly involved in intracellular transport and intestinal absorption of lipids. FABP2 has been considered a marker of mucosal injury and ischemia and serum I-FABP level is used as a tissue damage indicator. In addition, it is a marker of differentiated intestinal epithelial cells.

实验方案

Product Specific Protocols
WB protocol for FABP2 antibody 67691-1-IgDownload protocol
IHC protocol for FABP2 antibody 67691-1-IgDownload protocol
IF protocol for FABP2 antibody 67691-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Anti-Proteolytic Peptide R7I Protects the Intestinal Barrier and Alleviates Fatty Acid Malabsorption in Salmonella typhimurium-Infected Mice

Authors - Yunzhe Su
View Article