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  • Featured Product
  • KD/KO Validated

PPT1 Monoclonal antibody

PPT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A3

Cat No : 67699-1-Ig

Print datasheet

Synonyms

CLN1, INCL, Palmitoyl protein hydrolase 1, PPT, PPT 1, PPT1

验证数据展示

  • WB analysis using 67699-1-Ig

    WB analysis using 67699-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67699-1-Ig

    WB analysis of A549 using 67699-1-Ig

    WB result of PPT1 antibody (67699-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells.

  • WB analysis using 67699-1-Ig

    WB analysis using 67699-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, pig heart tissue, pig brain tissue, LNCap cells, HeLa cells, Jurkat cells, K-562 cells, pig cerebellum tissue, rabbit brain tissue, rabbit cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67699-1-Ig targets PPT1 in WB, ELISA applications and shows reactivity with Human, Pig, Rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Pig, Rabbit
Immunogen PPT1 fusion protein Ag30550 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name palmitoyl-protein thioesterase 1
Synonyms CLN1, INCL, Palmitoyl protein hydrolase 1, PPT, PPT 1, PPT1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight32-34 kda
GenBank Accession NumberBC008426
Gene Symbol PPT1
Gene ID (NCBI) 5538
RRIDAB_2882891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50897
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).

实验方案

Product Specific Protocols
WB protocol for PPT1 antibody 67699-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols