CEP290 Monoclonal antibody
CEP290 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rat, mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
2C10G2
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HSC-T6 cells, HeLa cells, NCCIT cells, K-562 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67716-1-Ig targets CEP290 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human, rat, mouse |
| Immunogen | CEP290 fusion protein Ag17457 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | centrosomal protein 290kDa |
| Synonyms | 3H11Ag, BBS14, Cancer/testis antigen 87, centrosomal protein 290kDa, Centrosomal protein of 290 kDa, CEP290, CT87, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, Nephrocystin 6, rd16, SLSN6, Tumor antigen se2 2 |
| Calculated Molecular Weight | 2479 aa, 290 kDa |
| Observed Molecular Weight | 290 kDa |
| GenBank Accession Number | BC008641 |
| Gene Symbol | CEP290 |
| Gene ID (NCBI) | 80184 |
| RRID | AB_2918499 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O15078 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP290 antibody 67716-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
