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MARS Monoclonal antibody

MARS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E3B4

Cat no : 67739-1-Ig

Print datasheet

Synonyms

FLJ35667, MARS, Methionine tRNA ligase, methionyl tRNA synthetase, METRS, MTRNS

验证数据展示

  • WB analysis using 67739-1-Ig

    WB analysis using 67739-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 67739-1-Ig

    WB analysis of A431 using 67739-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67739-1-Ig (MARS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67739-1-Ig

    IF Staining of HepG2 using 67739-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MARS antibody (67739-1-Ig, Clone: 3E3B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MARS1 Ready-To-Use IHC Kit. MARS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67739-1-Ig targets MARS in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen MARS fusion protein Ag6961 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name methionyl-tRNA synthetase
Synonyms FLJ35667, MARS, Methionine tRNA ligase, methionyl tRNA synthetase, METRS, MTRNS
Calculated Molecular Weight 101 kDa
Observed Molecular Weight 97-101 kDa
GenBank Accession NumberBC002384
Gene Symbol MARS
Gene ID (NCBI) 4141
RRIDAB_2918508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56192
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MARS antibody 67739-1-IgDownload protocol
IF protocol for MARS antibody 67739-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols