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验证数据展示

  • WB analysis using 67805-1-Ig

    WB analysis using 67805-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67805-1-Ig (USP11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67805-1-Ig

    WB analysis of HEK-293 using 67805-1-Ig

    WB result of USP11 antibody (67805-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP11 transfected HEK-293 cells.

  • IF Staining of HepG2 using 67805-1-Ig

    IF Staining of HepG2 using 67805-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP11 antibody (67805-1-Ig, Clone: 2G6E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human stomach cancer using 67805-1-Ig

    IHC staining of human stomach cancer using 67805-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USP11 antibody included
  • IHC staining of rat testis using 67805-1-Ig

    IHC staining of rat testis using 67805-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67805-1-Ig (USP11 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with USP11 antibody included
  • Featured Product
  • KD/KO验证

USP11 Monoclonal antibody

USP11 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67805-1-Ig

产品说明书

CloneNo. 2G6E7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Ubiquitin-specific-processing protease 11, Ubiquitin thiolesterase 11, Ubiquitin thioesterase 11, Ubiquitin carboxyl-terminal hydrolase 11, EC:3.4.19.12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, Ramos cells, Daudi cells
Positive IHC detected inrat testis tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy USP11 Ready-To-Use IHC Kit. USP11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67805-1-Ig targets USP11 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 USP11 fusion protein Ag19058 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 11
别名 Ubiquitin-specific-processing protease 11, Ubiquitin thiolesterase 11, Ubiquitin thioesterase 11, Ubiquitin carboxyl-terminal hydrolase 11, EC:3.4.19.12
计算分子量 963 aa, 110 kDa
观测分子量110 kDa
GenBank蛋白编号BC000350
基因名称 USP11
Gene ID (NCBI) 8237
RRIDAB_2918568
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP51784
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

实验方案

Product Specific Protocols
WB protocol for USP11 antibody 67805-1-IgDownload protocol
IHC protocol for USP11 antibody 67805-1-IgDownload protocol
IF protocol for USP11 antibody 67805-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols