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E2F4 Monoclonal antibody

E2F4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D1E1

Cat No : 67812-1-Ig

Print datasheet

Synonyms

E2F 4, E2F4, Transcription factor E2F4

验证数据展示

  • WB analysis using 67812-1-Ig

    WB analysis using 67812-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 67812-1-Ig

    WB analysis using 67812-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

经过测试的应用

Positive WB detected inLNCaP cells, HSC-T6 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67812-1-Ig targets E2F4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen E2F4 fusion protein Ag28533 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name E2F transcription factor 4, p107/p130-binding
Synonyms E2F 4, E2F4, Transcription factor E2F4
Calculated Molecular Weight 44 kDa
GenBank Accession NumberBC033180
Gene Symbol E2F4
Gene ID (NCBI) 1874
RRIDAB_2918575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16254
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.

实验方案

Product Specific Protocols
WB protocol for E2F4 antibody 67812-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols